TY - JOUR
T1 - A novel streptomyces restriction endonuclease, Sse1825I, cleaving at 5′-GG/GWCCC-3′
AU - Nomura, Yoshiko
AU - Ishino, Yoshizumi
AU - Kimizuka, Fusao
AU - Kato, Ikunoshin
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1995/5/19
Y1 - 1995/5/19
N2 - We isolated and characterized from a Streptomyces species a new class-II restriction endonuclease, which recognizes the palindromic heptanucleotide sequence 5′-G G↓G W C C C 3′-C C C W G↑G G (where W = A or T) and cleaves double-stranded DNA after the second G in this sequence This Sse18251 enzyme cleaves phage λ DNA at one site, adenovirus type 2 DNA at eight sites, but does not cleave pBR322, SV40, CoIE1, pUC18 and pUC19, and replicative forms of M13mpl8 and M13mpl9, and ΦX174 DNAs.
AB - We isolated and characterized from a Streptomyces species a new class-II restriction endonuclease, which recognizes the palindromic heptanucleotide sequence 5′-G G↓G W C C C 3′-C C C W G↑G G (where W = A or T) and cleaves double-stranded DNA after the second G in this sequence This Sse18251 enzyme cleaves phage λ DNA at one site, adenovirus type 2 DNA at eight sites, but does not cleave pBR322, SV40, CoIE1, pUC18 and pUC19, and replicative forms of M13mpl8 and M13mpl9, and ΦX174 DNAs.
UR - http://www.scopus.com/inward/record.url?scp=0029016158&partnerID=8YFLogxK
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U2 - 10.1016/0378-1119(95)00119-Q
DO - 10.1016/0378-1119(95)00119-Q
M3 - Article
C2 - 7607520
AN - SCOPUS:0029016158
SN - 0378-1119
VL - 157
SP - 323
EP - 324
JO - Gene
JF - Gene
IS - 1-2
ER -