A novel mechanism in suppression of erythropoiesis during inflammation: A crucial role of RCAS1

Youko Suehiro, Koichiro Muta, Manabu Nakashima, Yasunobu Abe, Motoaki Shiratsuchi, Satoshi Shiokawa, Shoichiro Ikuyama, Yasuji Yoshikawa, Takeshi Watanabe, Junji Nishimura

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


A novel human tumor-associated antigen, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), induces apoptosis in normal human erythroid progenitor cells, which express putative RCAS1 receptors. In the present study, we investigated a possible role of RCAS1 produced by human peripheral blood monocytes (CD14-positive cells) and monocyte-derived macrophages. RCAS1 was immunohistochemically detected in monocytes as well as macrophages. When macrophages were stimulated with lipopolysaccharide (LPS), the expression of RCAS1 was remarkably enhanced. An increased production of RCAS1 mRNA was observed in LPS-stimulated macrophages by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Soluble RCAS1 molecules were only detected in the culture supernatants obtained from LPS-stimulated macrophages. Moreover, LPS-stimulated macrophages induced cell death of erythroid progenitor cells through RCAS1 production. These results suggest that macrophages may negatively regulate erythropoiesis at least in part through the production of RCAS1 molecules, and this may contribute to the pathogenesis of the anemia seen in patients with inflammatory disorders.

Original languageEnglish
Pages (from-to)365-373
Number of pages9
JournalEuropean Journal of Haematology
Issue number5
Publication statusPublished - May 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Hematology


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