@inproceedings{152e8729d289444da10692738217fbc4,
title = "A novel fiber-free technique for brain activity imaging in multiple freely behaving mice",
abstract = "Brain functions and related psychiatric disorders have been investigated by recording electrophysiological field potential. When recording it, a conventional method requires fiber-based apparatus connected to the brain, which however hampers the simultaneous measurement in multiple animals (e.g. by a tangle of fibers). Here, we propose a fiber-free recording technique in conjunction with a ratiometric bioluminescent voltage indicator. Our method allows investigation of electrophysiological filed potential dynamics in multiple freely behaving animals simultaneously over a long time period. Therefore, this fiber-free technique opens up the way to investigate a new mechanism of brain function that governs social behaviors and animal-to-animal interaction.",
author = "Shigenori Inagaki and Masakazu Agetsuma and Takeharu Nagai",
note = "Funding Information: T.N. was supported by MEXT (No. 3306, No. 23115003, No. 23115001, No. 26251018), the JST-SENTAN program, JSPS Core-to-Core Program, A. Advanced Research Networks, the Uehara Memorial Foundation, and the Naito Foundation. S.I. was supported by Grant-in-Aid for JSPS Research Fellow (No. 16J00111) and “Program for Leading Graduate Schools”. M.A was supported by MEXT (No. 15K18341). We thank Akihiro Yamanaka (Nagoya University, RIEM) for providing us with an AAV purification method and Mitsuhiro Iwaki (RIKEN QBiC) for lending us a C8600-05 GaAsP image intensifier unit. We also thank the Bionanophotonics Consortium (BNPC) for assistance in experiments using microscopy. Publisher Copyright: {\textcopyright} COPYRIGHT SPIE.; Optogenetics and Optical Manipulation 2018 ; Conference date: 27-01-2018 Through 28-01-2018",
year = "2018",
doi = "10.1117/12.2302396",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Jansen, {E. Duco} and Mohanty, {Samarendra K.} and Thakor, {Nitish V.} and Thakor, {Nitish V.}",
booktitle = "Optogenetics and Optical Manipulation 2018",
address = "United States",
}