In the present study, we have demonstrated the fabrication of novel enzymatic glucose biosensor using glucose oxidase (GOD) as a model enzyme which has been immobilized onto the graphene (GF) and cobalt oxide nanoparticles (Co3O4-NPs) composite modified electrode. The GF/Co 3O4-NPs composite was prepared by hydrothermal method and characterized by using scanning electron microscopy, X-ray diffraction and elemental analysis. The GOD immobilized GF/Co3O4-NPs modified electrode shows a well defined redox behaviour indicating the reversible proton and electron transfer reaction of GOD. A heterogeneous electron transfer rate constant (Ks) of immobilized GOD has been calculated to be 3.52 s-1 which is much higher than that of GOD immobilized GF supports. The fast electron transfer of GOD is attributed to the excellent biocompatibility of Co3O4-NPs and high conductivity of the GF. The fabricated glucose biosensor exhibits a wider linear response for glucose from 0.5 mM to 16.5 mM with the sensitivity of 13.52 μA mM-1 cm-2. In addition, a non-enzymatic H 2O2 sensor has been further developed using GF/Co 3O4-NPs composite modified electrode. The GF/Co 3O4-NPs composite electrode shows an excellent electrocatalytic activity towards H2O2 with the response time of <10 s. The H2O2 response at GF/Co 3O4-NPs composite modified electrode displays a linear response ranging from 0.2 to 211.5 μM with a limit of detection of 0.06 μM.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry