TY - JOUR
T1 - A novel dnaK operon from Porphyromonas gingivalis
AU - Yoshida, Akihiro
AU - Nakano, Yoshio
AU - Yamashita, Yoshihisa
AU - Oho, Takahiko
AU - Shibata, Yukie
AU - Ohishi, Masamichi
AU - Koga, Toshihiko
N1 - Funding Information:
This work was supported in part by Grants-in-aid of Scientific Research number 10307054 and 08457572 from the Ministry of Education, Science, Sports and Culture, Tokyo, Japan and by a research grant from the Fund for Comprehensive Research on Aging and Health.
PY - 1999/3/12
Y1 - 1999/3/12
N2 - The nucleotide sequence of the dnaK operon cloned from Porphyromonas gingivalis revealed that the operon does not contain homologues of either dnaJ or grpE. However, there were two genes which encode small heat shock proteins immediately downstream from the dnaK and they were transcribed together with dnaK as one unit. The ATPase activity of the P. gingivalis DnaK was synergistically stimulated up to 40-fold in the simultaneous presence of Escherichia coli DnaJ and GrpE. These results suggest that the DnaK homologue of P. gingivalis, with its unique genetic structure and evolutionary features, works as a member of the DnaK chaperone system. Copyright (C) 1999 Federation of European Biochemical Societies.
AB - The nucleotide sequence of the dnaK operon cloned from Porphyromonas gingivalis revealed that the operon does not contain homologues of either dnaJ or grpE. However, there were two genes which encode small heat shock proteins immediately downstream from the dnaK and they were transcribed together with dnaK as one unit. The ATPase activity of the P. gingivalis DnaK was synergistically stimulated up to 40-fold in the simultaneous presence of Escherichia coli DnaJ and GrpE. These results suggest that the DnaK homologue of P. gingivalis, with its unique genetic structure and evolutionary features, works as a member of the DnaK chaperone system. Copyright (C) 1999 Federation of European Biochemical Societies.
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U2 - 10.1016/S0014-5793(99)00237-9
DO - 10.1016/S0014-5793(99)00237-9
M3 - Article
C2 - 10100860
AN - SCOPUS:0033039866
SN - 0014-5793
VL - 446
SP - 287
EP - 291
JO - FEBS Letters
JF - FEBS Letters
IS - 2-3
ER -