A new small molecule DoNA binding to CAG repeat RNA

Qingwen Chen; Takeshi Yamada; Koichi Miyagawa; Asako Murata; Mitsuo Shoji; Kazuhiko Nakatani

doi:10.1016/j.bmc.2023.117580

A new small molecule DoNA binding to CAG repeat RNA

Qingwen Chen, Takeshi Yamada, Koichi Miyagawa, Asako Murata, Mitsuo Shoji, Kazuhiko Nakatani

Quantum Materials Science

Research output: Contribution to journal › Article › peer-review

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Abstract

We here report a new molecule DoNA binding to a CAG repeat RNA. DoNA is a dimer of the NA molecule that we previously reported. NA binds with high affinity to a CAG repeat DNA but not significantly to a CAG repeat RNA. Binding analyses using SPR and CSI-TOF MS indicated a significant increase in the affinity of DoNA to a single stranded CAG repeat RNA compared to NA. Systematic investigation of the RNA motifs bound by DoNA using hairpin RNA models revealed that DoNA binds to the CAG units at overhang and terminal positions, and notably, it binds to the structurally flexible internal and hairpin loop region.

Original language	English
Article number	117580
Journal	Bioorganic and Medicinal Chemistry
Volume	98
DOIs	https://doi.org/10.1016/j.bmc.2023.117580
Publication status	Published - Jan 15 2024

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmc.2023.117580

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title = "A new small molecule DoNA binding to CAG repeat RNA",

abstract = "We here report a new molecule DoNA binding to a CAG repeat RNA. DoNA is a dimer of the NA molecule that we previously reported. NA binds with high affinity to a CAG repeat DNA but not significantly to a CAG repeat RNA. Binding analyses using SPR and CSI-TOF MS indicated a significant increase in the affinity of DoNA to a single stranded CAG repeat RNA compared to NA. Systematic investigation of the RNA motifs bound by DoNA using hairpin RNA models revealed that DoNA binds to the CAG units at overhang and terminal positions, and notably, it binds to the structurally flexible internal and hairpin loop region.",

keywords = "CAG repeats, DoNA, RNA binding small molecule",

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T1 - A new small molecule DoNA binding to CAG repeat RNA

AU - Chen, Qingwen

AU - Yamada, Takeshi

AU - Miyagawa, Koichi

AU - Murata, Asako

AU - Shoji, Mitsuo

AU - Nakatani, Kazuhiko

PY - 2024/1/15

Y1 - 2024/1/15

N2 - We here report a new molecule DoNA binding to a CAG repeat RNA. DoNA is a dimer of the NA molecule that we previously reported. NA binds with high affinity to a CAG repeat DNA but not significantly to a CAG repeat RNA. Binding analyses using SPR and CSI-TOF MS indicated a significant increase in the affinity of DoNA to a single stranded CAG repeat RNA compared to NA. Systematic investigation of the RNA motifs bound by DoNA using hairpin RNA models revealed that DoNA binds to the CAG units at overhang and terminal positions, and notably, it binds to the structurally flexible internal and hairpin loop region.

AB - We here report a new molecule DoNA binding to a CAG repeat RNA. DoNA is a dimer of the NA molecule that we previously reported. NA binds with high affinity to a CAG repeat DNA but not significantly to a CAG repeat RNA. Binding analyses using SPR and CSI-TOF MS indicated a significant increase in the affinity of DoNA to a single stranded CAG repeat RNA compared to NA. Systematic investigation of the RNA motifs bound by DoNA using hairpin RNA models revealed that DoNA binds to the CAG units at overhang and terminal positions, and notably, it binds to the structurally flexible internal and hairpin loop region.

KW - CAG repeats

KW - DoNA

KW - RNA binding small molecule

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JO - Bioorganic and Medicinal Chemistry

JF - Bioorganic and Medicinal Chemistry

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ER -

A new small molecule DoNA binding to CAG repeat RNA

Abstract

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