TY - JOUR
T1 - A new method for comprehensive bird's-eye analysis of the surgically excised internal limiting membrane
AU - Hisatomi, Toshio
AU - Enaida, Hiroshi
AU - Sakamoto, Taiji
AU - Kagimoto, Tadahisa
AU - Ueno, Akifumi
AU - Nakamura, Takao
AU - Hata, Yasuaki
AU - Ishibashi, Tatsuro
N1 - Funding Information:
Supported in part by grants-in-aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of the Japanese Government (No. 16791052) and the Japan National Society for the Prevention of Blindness (Tokyo).
PY - 2005/6
Y1 - 2005/6
N2 - PURPOSE: To investigate the role of cells and extracellular matrix on the internal limiting membrane (ILM), we demonstrated a new method for a comprehensive bird's-eye analysis of surgically excised ILM. DESIGN: Laboratory investigation. METHODS: The ILM of an idiopathic epiretinal membrane was fixed and spread out onto a glass slide, using fine needles under a biomicroscope. The expanded ILM was analyzed by light microscopy, immunohistochemistry, and scanning electron microscopy. RESULTS: The excised ILM could be unfolded and spread out as a flat sheet onto the glass slide. Immunohistochemistry revealed that most migrating cells were glial cells. Scanning electronmicroscopy revealed that the vitreous surface of the ILM was smooth, in contrast to the rough retinal surface. The ILM showed abundant cellular migration as a continuous stratified cellular sheet. CONCLUSIONS: This new bird's-eye-view observation of excised ILM enables us to carry out comprehensive analysis of cellular distribution from a temporal and spatial perspective.
AB - PURPOSE: To investigate the role of cells and extracellular matrix on the internal limiting membrane (ILM), we demonstrated a new method for a comprehensive bird's-eye analysis of surgically excised ILM. DESIGN: Laboratory investigation. METHODS: The ILM of an idiopathic epiretinal membrane was fixed and spread out onto a glass slide, using fine needles under a biomicroscope. The expanded ILM was analyzed by light microscopy, immunohistochemistry, and scanning electron microscopy. RESULTS: The excised ILM could be unfolded and spread out as a flat sheet onto the glass slide. Immunohistochemistry revealed that most migrating cells were glial cells. Scanning electronmicroscopy revealed that the vitreous surface of the ILM was smooth, in contrast to the rough retinal surface. The ILM showed abundant cellular migration as a continuous stratified cellular sheet. CONCLUSIONS: This new bird's-eye-view observation of excised ILM enables us to carry out comprehensive analysis of cellular distribution from a temporal and spatial perspective.
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U2 - 10.1016/j.ajo.2004.11.051
DO - 10.1016/j.ajo.2004.11.051
M3 - Article
C2 - 15953453
AN - SCOPUS:20444467652
SN - 0002-9394
VL - 139
SP - 1121
EP - 1122
JO - American journal of ophthalmology
JF - American journal of ophthalmology
IS - 6
ER -