A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip

Yong Zhang; Guichen Ping; Bingmei Zhu; Noritada Kaji; Manabu Tokeshi; Yoshinobu Baba

A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip

Yong Zhang, Guichen Ping, Bingmei Zhu, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to conference › Paper › peer-review

Abstract

The glycosaminoglycans (GAGs) labeled with 2-aminoacridone, hydrochloride (AMAC) were found to be prevented from adsorption to the surface of polymehylmethacrylate (PMMA) microchips by dynamic coating microchannel with methyl cellulose (MC). To obtain sufficient resolution of mono-sulfate GAG disaccharide isomers on microchip electrophoresis (MCE), buffer pH, β-cyclodextrin (β-CD), and 1,4-dioxane (DO) were systematically investigated. We found that the application of 1,4-dioxane (DO) dramatically improved the resolution of the analytes. ΔDi-triS, ΔDi-UA2S, ΔDi-4S, and ΔDi-6S were baseline separated within 75 sec. The theoretical plate was in the range of 5×10⁵-1×10 ⁶ m^-1.

Original language	English
Pages	395-397
Number of pages	3
Publication status	Published - 2006
Externally published	Yes
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	11/5/06 → 11/9/06

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Zhang, Y., Ping, G., Zhu, B., Kaji, N., Tokeshi, M., & Baba, Y. (2006). A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip. 395-397. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip. / Zhang, Yong; Ping, Guichen; Zhu, Bingmei et al.
2006. 395-397 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

Zhang, Y, Ping, G, Zhu, B, Kaji, N, Tokeshi, M & Baba, Y 2006, 'A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip', Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 11/5/06 - 11/9/06 pp. 395-397.

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title = "A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip",

abstract = "The glycosaminoglycans (GAGs) labeled with 2-aminoacridone, hydrochloride (AMAC) were found to be prevented from adsorption to the surface of polymehylmethacrylate (PMMA) microchips by dynamic coating microchannel with methyl cellulose (MC). To obtain sufficient resolution of mono-sulfate GAG disaccharide isomers on microchip electrophoresis (MCE), buffer pH, β-cyclodextrin (β-CD), and 1,4-dioxane (DO) were systematically investigated. We found that the application of 1,4-dioxane (DO) dramatically improved the resolution of the analytes. ΔDi-triS, ΔDi-UA2S, ΔDi-4S, and ΔDi-6S were baseline separated within 75 sec. The theoretical plate was in the range of 5×105-1×10 6 m-1.",

author = "Yong Zhang and Guichen Ping and Bingmei Zhu and Noritada Kaji and Manabu Tokeshi and Yoshinobu Baba",

year = "2006",

language = "English",

pages = "395--397",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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T1 - A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip

AU - Zhang, Yong

AU - Ping, Guichen

AU - Zhu, Bingmei

AU - Kaji, Noritada

AU - Tokeshi, Manabu

AU - Baba, Yoshinobu

PY - 2006

Y1 - 2006

N2 - The glycosaminoglycans (GAGs) labeled with 2-aminoacridone, hydrochloride (AMAC) were found to be prevented from adsorption to the surface of polymehylmethacrylate (PMMA) microchips by dynamic coating microchannel with methyl cellulose (MC). To obtain sufficient resolution of mono-sulfate GAG disaccharide isomers on microchip electrophoresis (MCE), buffer pH, β-cyclodextrin (β-CD), and 1,4-dioxane (DO) were systematically investigated. We found that the application of 1,4-dioxane (DO) dramatically improved the resolution of the analytes. ΔDi-triS, ΔDi-UA2S, ΔDi-4S, and ΔDi-6S were baseline separated within 75 sec. The theoretical plate was in the range of 5×105-1×10 6 m-1.

AB - The glycosaminoglycans (GAGs) labeled with 2-aminoacridone, hydrochloride (AMAC) were found to be prevented from adsorption to the surface of polymehylmethacrylate (PMMA) microchips by dynamic coating microchannel with methyl cellulose (MC). To obtain sufficient resolution of mono-sulfate GAG disaccharide isomers on microchip electrophoresis (MCE), buffer pH, β-cyclodextrin (β-CD), and 1,4-dioxane (DO) were systematically investigated. We found that the application of 1,4-dioxane (DO) dramatically improved the resolution of the analytes. ΔDi-triS, ΔDi-UA2S, ΔDi-4S, and ΔDi-6S were baseline separated within 75 sec. The theoretical plate was in the range of 5×105-1×10 6 m-1.

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A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip

Abstract

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All Science Journal Classification (ASJC) codes

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