A micropatterned carbohydrate display for tissue engineering by self-assembly of heparin

Hajime Sato; Yoshiko Miura; Nagahiro Saito; Kazukiyo Kobayashi; Osamu Takai

doi:10.1016/j.susc.2007.04.066

A micropatterned carbohydrate display for tissue engineering by self-assembly of heparin

Hajime Sato, Yoshiko Miura, Nagahiro Saito, Kazukiyo Kobayashi, Osamu Takai

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A novel strategy to construct a fibroblast scaffold on substrates has been demonstrated via top-down photolithography and the subsequent bottom-up processes of molecular self-assembly and molecular recognition. 3-Aminopropyltrimethoxysilane (APS) self-assembled monolayer was micropatterned by photolithography. An anionic polysaccharide heparin was adsorbed selectively on the cationic APS region of the micropatterned substrate. Basic fibroblast growth factor (bFGF) was selectively bound to the displayed heparin region and then micropatterned cultivation of fibroblast cells was successful on the bFGF-heparin-APS substrate.

Original language	English
Pages (from-to)	3871-3875
Number of pages	5
Journal	Surface Science
Volume	601
Issue number	18
DOIs	https://doi.org/10.1016/j.susc.2007.04.066
Publication status	Published - Sept 15 2007
Externally published	Yes

All Science Journal Classification (ASJC) codes

Condensed Matter Physics
Surfaces and Interfaces
Surfaces, Coatings and Films
Materials Chemistry

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10.1016/j.susc.2007.04.066

Cite this

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title = "A micropatterned carbohydrate display for tissue engineering by self-assembly of heparin",

abstract = "A novel strategy to construct a fibroblast scaffold on substrates has been demonstrated via top-down photolithography and the subsequent bottom-up processes of molecular self-assembly and molecular recognition. 3-Aminopropyltrimethoxysilane (APS) self-assembled monolayer was micropatterned by photolithography. An anionic polysaccharide heparin was adsorbed selectively on the cationic APS region of the micropatterned substrate. Basic fibroblast growth factor (bFGF) was selectively bound to the displayed heparin region and then micropatterned cultivation of fibroblast cells was successful on the bFGF-heparin-APS substrate.",

author = "Hajime Sato and Yoshiko Miura and Nagahiro Saito and Kazukiyo Kobayashi and Osamu Takai",

note = "Funding Information: This work was supported by the 21st Century COE Program “Nature-guided Materials Processing” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) and by the Industrial Technology Research Grant Program in 2003–2005 of the New Energy and Industrial Technology Development Organization (NEDO).",

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doi = "10.1016/j.susc.2007.04.066",

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T1 - A micropatterned carbohydrate display for tissue engineering by self-assembly of heparin

AU - Sato, Hajime

AU - Miura, Yoshiko

AU - Saito, Nagahiro

AU - Kobayashi, Kazukiyo

AU - Takai, Osamu

N1 - Funding Information: This work was supported by the 21st Century COE Program “Nature-guided Materials Processing” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) and by the Industrial Technology Research Grant Program in 2003–2005 of the New Energy and Industrial Technology Development Organization (NEDO).

PY - 2007/9/15

Y1 - 2007/9/15

N2 - A novel strategy to construct a fibroblast scaffold on substrates has been demonstrated via top-down photolithography and the subsequent bottom-up processes of molecular self-assembly and molecular recognition. 3-Aminopropyltrimethoxysilane (APS) self-assembled monolayer was micropatterned by photolithography. An anionic polysaccharide heparin was adsorbed selectively on the cationic APS region of the micropatterned substrate. Basic fibroblast growth factor (bFGF) was selectively bound to the displayed heparin region and then micropatterned cultivation of fibroblast cells was successful on the bFGF-heparin-APS substrate.

AB - A novel strategy to construct a fibroblast scaffold on substrates has been demonstrated via top-down photolithography and the subsequent bottom-up processes of molecular self-assembly and molecular recognition. 3-Aminopropyltrimethoxysilane (APS) self-assembled monolayer was micropatterned by photolithography. An anionic polysaccharide heparin was adsorbed selectively on the cationic APS region of the micropatterned substrate. Basic fibroblast growth factor (bFGF) was selectively bound to the displayed heparin region and then micropatterned cultivation of fibroblast cells was successful on the bFGF-heparin-APS substrate.

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JO - Surface Science

JF - Surface Science

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ER -

A micropatterned carbohydrate display for tissue engineering by self-assembly of heparin

Abstract

All Science Journal Classification (ASJC) codes

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