A microfluidic molecular tracking system detects a time-to-search DNA sequence to be cleaved by restriction enzymes

D. Onoshima; N. Kaji; M. Tokeshi; Y. Baba

A microfluidic molecular tracking system detects a time-to-search DNA sequence to be cleaved by restriction enzymes

D. Onoshima, N. Kaji, M. Tokeshi, Y. Baba

Research output: Contribution to conference › Paper › peer-review

Abstract

A microfluidic analytical system, which uses simple technique to pick reacting DNA molecules out of a crowd of molecules during enzymatic reaction, was developed on the basis of single-molecule imaging. Restriction enzymes EcoR I and Not I with their target and non-target DNA were analyzed. With this system, we measured the duration difference of restriction-site-searching by these enzymes at the single-molecule level.

Original language	English
Pages	18-20
Number of pages	3
Publication status	Published - 2008
Externally published	Yes
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

A microfluidic molecular tracking system detects a time-to-search DNA sequence to be cleaved by restriction enzymes. / Onoshima, D.; Kaji, N.; Tokeshi, M. et al.
2008. 18-20 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

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abstract = "A microfluidic analytical system, which uses simple technique to pick reacting DNA molecules out of a crowd of molecules during enzymatic reaction, was developed on the basis of single-molecule imaging. Restriction enzymes EcoR I and Not I with their target and non-target DNA were analyzed. With this system, we measured the duration difference of restriction-site-searching by these enzymes at the single-molecule level.",

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AU - Onoshima, D.

AU - Kaji, N.

AU - Tokeshi, M.

AU - Baba, Y.

PY - 2008

Y1 - 2008

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AB - A microfluidic analytical system, which uses simple technique to pick reacting DNA molecules out of a crowd of molecules during enzymatic reaction, was developed on the basis of single-molecule imaging. Restriction enzymes EcoR I and Not I with their target and non-target DNA were analyzed. With this system, we measured the duration difference of restriction-site-searching by these enzymes at the single-molecule level.

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M3 - Paper

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T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

A microfluidic molecular tracking system detects a time-to-search DNA sequence to be cleaved by restriction enzymes

Abstract

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All Science Journal Classification (ASJC) codes

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