TY - JOUR
T1 - A methylation imprint mark in the mouse imprinted gene Grf1/Cdc25Mm locus shares a common feature with the U2afbp-rs gene
T2 - An association with a short tandem repeat and a hypermethylated region
AU - Shibata, Hideo
AU - Yoda, Yoshika
AU - Kato, Reiko
AU - Ueda, Takayuki
AU - Kamiya, Mamoru
AU - Hiraiwa, Noriko
AU - Yoshiki, Atsushi
AU - Plass, Christoph
AU - Pearsall, R. Scott
AU - Held, William A.
AU - Muramatsu, Masami
AU - Sasaki, Hiroyuki
AU - Kusakabe, Moriaki
AU - Hayashizaki, Yoshihide
N1 - Funding Information:
We thank Drs. S. Takada, T. Arima, Y. Okazaki, and T. Akama-Ohsumi for valuable discussion; Drs. N. Sasaki, P. Carninci, M. Ta-teno-Sugita, J. Kawai, S. Nagaoka, and M. Itoh for critical reading and comments on the manuscript. We also thank Miss M. Itoh and N. Kikuchi for technical assistance. This work was supported by a Core Research of Evolutional Science and Technology Program from JST and Special Coordination Funds and a Research Grant for the Genome Exploration Research Project from the Science and Technology Agency of the Japanese Government to Y.H. This study was also supported by a grant-in-aid for a second Term Comprehensive 10-Year Strategy for Cancer Control from the Ministry of Health and Welfare, by a grant-in-aid for Scienti®c Research on Priority Areas and Human Genome Program from The Ministry of Education and Culture, Japan, to Y.H., and also by National Institutes of Health Grant R01 GM51881-02 to W.A.H.
PY - 1998/4/1
Y1 - 1998/4/1
N2 - We identified a sperm-specific methylation imprint mark (Site II) associated with a short tandem repeat sequence and a site/region methylated in both gametes (Site I) in the Grf1 locus on mouse chromosome 9, which shared a common feature with the U2afbp-rs gene. Sites or regions of gamete- specific methylation in imprinted genes are strong candidates for carrying information regarding the parental origin of alleles. The gamete-specific methylation pattern of Sites I and II was conserved after fertilization, but attained the somatic cell pattern by the blastocyst stage. In primordial germ cells, Site I was methylated, but Site II was unmethylated in both male and female embryos, suggesting that the sperm-specific methylation imprint mark in Site II was established during spermatogenesis. These common features in methylation imprint regions may be a clue to identifying regions carrying primary information for the imprinting regulation.
AB - We identified a sperm-specific methylation imprint mark (Site II) associated with a short tandem repeat sequence and a site/region methylated in both gametes (Site I) in the Grf1 locus on mouse chromosome 9, which shared a common feature with the U2afbp-rs gene. Sites or regions of gamete- specific methylation in imprinted genes are strong candidates for carrying information regarding the parental origin of alleles. The gamete-specific methylation pattern of Sites I and II was conserved after fertilization, but attained the somatic cell pattern by the blastocyst stage. In primordial germ cells, Site I was methylated, but Site II was unmethylated in both male and female embryos, suggesting that the sperm-specific methylation imprint mark in Site II was established during spermatogenesis. These common features in methylation imprint regions may be a clue to identifying regions carrying primary information for the imprinting regulation.
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U2 - 10.1006/geno.1998.5218
DO - 10.1006/geno.1998.5218
M3 - Article
C2 - 9570946
AN - SCOPUS:0032053874
SN - 0888-7543
VL - 49
SP - 30
EP - 37
JO - Genomics
JF - Genomics
IS - 1
ER -