A kinetic expression for hydrolysis of soluble starch by glucoamylase

Koichiro Kusunoki; Koei Kawakami; Fumihide Shiraishi; Keiichi Kato; Mayumi Kai

doi:10.1002/bit.260240208

A kinetic expression for hydrolysis of soluble starch by glucoamylase

Koichiro Kusunoki, Koei Kawakami, Fumihide Shiraishi, Keiichi Kato, Mayumi Kai

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

As the hydrolysis of starch by glucoamylase proceeds with stepwise removal of glucose units from the nonreducing ends of the starch chain, the number of available substrate molecules is essentially unchanged in the course of the degradation. In view of this aspect, a simple practical kinetic expression, which consists of a modified Michaelis–Menten form with product inhibition, is presented for the hydrolysis of soluble starch. It is assumed that the values of kinetic parameters V_m and K_m vary linearly from the values for starch toward those for maltose. The applicability of this kinetic expression is verified through the simulation with the experimental results for the hydrolysis of two soluble starches with different average molecular weights of 3 × 10⁴ and 3 × 10⁶.

Original language	English
Pages (from-to)	347-354
Number of pages	8
Journal	Biotechnology and Bioengineering
Volume	24
Issue number	2
DOIs	https://doi.org/10.1002/bit.260240208
Publication status	Published - Feb 1982
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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abstract = "As the hydrolysis of starch by glucoamylase proceeds with stepwise removal of glucose units from the nonreducing ends of the starch chain, the number of available substrate molecules is essentially unchanged in the course of the degradation. In view of this aspect, a simple practical kinetic expression, which consists of a modified Michaelis–Menten form with product inhibition, is presented for the hydrolysis of soluble starch. It is assumed that the values of kinetic parameters Vm and Km vary linearly from the values for starch toward those for maltose. The applicability of this kinetic expression is verified through the simulation with the experimental results for the hydrolysis of two soluble starches with different average molecular weights of 3 × 104 and 3 × 106.",

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AU - Kusunoki, Koichiro

AU - Kawakami, Koei

AU - Shiraishi, Fumihide

AU - Kato, Keiichi

AU - Kai, Mayumi

PY - 1982/2

Y1 - 1982/2

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AB - As the hydrolysis of starch by glucoamylase proceeds with stepwise removal of glucose units from the nonreducing ends of the starch chain, the number of available substrate molecules is essentially unchanged in the course of the degradation. In view of this aspect, a simple practical kinetic expression, which consists of a modified Michaelis–Menten form with product inhibition, is presented for the hydrolysis of soluble starch. It is assumed that the values of kinetic parameters Vm and Km vary linearly from the values for starch toward those for maltose. The applicability of this kinetic expression is verified through the simulation with the experimental results for the hydrolysis of two soluble starches with different average molecular weights of 3 × 104 and 3 × 106.

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A kinetic expression for hydrolysis of soluble starch by glucoamylase

Abstract

All Science Journal Classification (ASJC) codes

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