TY - JOUR
T1 - A highly efficient method for concentrating DNA from river water by combined coagulation and foam separation
AU - Suzuki, Yoshihiro
AU - Imafuku, Yuki
AU - Nishiyama, Masateru
AU - Teranishi, Kotaro
AU - Jikumaru, Atsushi
AU - Nukazawa, Kei
AU - Ogura, Yoshitoshi
N1 - Publisher Copyright:
© 2019, © 2019 Taylor & Francis Group, LLC.
PY - 2019/12/12
Y1 - 2019/12/12
N2 - Both Escherichia coli and Enterococci were collected in foam within 7 min from 500 mL of bacteria-spiked water by coagulation and foam separation using ferric chloride and milk casein. These bacterial DNA isolated in the 100 µL of extract from the foam more than 87.5% recovery using the DNeasy PowerWater® Kit. To test this method with water from three natural rivers, 0.67–2.70 µg of DNA were concentrated in 100 µL of extract from 1,000 mL of river water. When the DNA extract was subjected to 16S rRNA gene sequencing analysis, information on the bacterial flora could be obtained.
AB - Both Escherichia coli and Enterococci were collected in foam within 7 min from 500 mL of bacteria-spiked water by coagulation and foam separation using ferric chloride and milk casein. These bacterial DNA isolated in the 100 µL of extract from the foam more than 87.5% recovery using the DNeasy PowerWater® Kit. To test this method with water from three natural rivers, 0.67–2.70 µg of DNA were concentrated in 100 µL of extract from 1,000 mL of river water. When the DNA extract was subjected to 16S rRNA gene sequencing analysis, information on the bacterial flora could be obtained.
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U2 - 10.1080/01496395.2019.1565774
DO - 10.1080/01496395.2019.1565774
M3 - Article
AN - SCOPUS:85060336638
SN - 0149-6395
VL - 54
SP - 3128
EP - 3134
JO - Separation Science and Technology (Philadelphia)
JF - Separation Science and Technology (Philadelphia)
IS - 18
ER -