TY - JOUR
T1 - A harmonized immunoassay with liquid chromatography-mass spectrometry analysis in egg allergen determination
AU - Nimata, Masaomi
AU - Okada, Hideki
AU - Kurihara, Kei
AU - Sugimoto, Tsukasa
AU - Honjoh, Tsutomu
AU - Kuroda, Kazuhiko
AU - Yano, Takeo
AU - Tachibana, Hirofumi
AU - Shoji, Masahiro
N1 - Publisher Copyright:
© 2017, Springer-Verlag GmbH Germany.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Food allergy is a serious health issue worldwide. Implementing allergen labeling regulations is extremely challenging for regulators, food manufacturers, and analytical kit manufacturers. Here we have developed an “amino acid sequence immunoassay” approach to ELISA. The new ELISA comprises of a monoclonal antibody generated via an analyte specific peptide antigen and sodium lauryl sulfate/sulfite solution. This combination enables the antibody to access the epitope site in unfolded analyte protein. The newly developed ELISA recovered 87.1%–106.4% ovalbumin from ovalbumin-incurred model processed foods, thereby demonstrating its applicability as practical egg allergen determination. Furthermore, the comparison of LC-MS/MS and the new ELISA, which targets the amino acid sequence conforming to the LC-MS/MS detection peptide, showed a good agreement. Consequently the harmonization of two methods was demonstrated. The complementary use of the new ELISA and LC-MS analysis can offer a wide range of practical benefits in terms of easiness, cost, accuracy, and efficiency in food allergen analysis. In addition, the new assay is attractive in respect to its easy antigen preparation and predetermined specificity. [Figure not available: see fulltext.].
AB - Food allergy is a serious health issue worldwide. Implementing allergen labeling regulations is extremely challenging for regulators, food manufacturers, and analytical kit manufacturers. Here we have developed an “amino acid sequence immunoassay” approach to ELISA. The new ELISA comprises of a monoclonal antibody generated via an analyte specific peptide antigen and sodium lauryl sulfate/sulfite solution. This combination enables the antibody to access the epitope site in unfolded analyte protein. The newly developed ELISA recovered 87.1%–106.4% ovalbumin from ovalbumin-incurred model processed foods, thereby demonstrating its applicability as practical egg allergen determination. Furthermore, the comparison of LC-MS/MS and the new ELISA, which targets the amino acid sequence conforming to the LC-MS/MS detection peptide, showed a good agreement. Consequently the harmonization of two methods was demonstrated. The complementary use of the new ELISA and LC-MS analysis can offer a wide range of practical benefits in terms of easiness, cost, accuracy, and efficiency in food allergen analysis. In addition, the new assay is attractive in respect to its easy antigen preparation and predetermined specificity. [Figure not available: see fulltext.].
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U2 - 10.1007/s00216-017-0721-z
DO - 10.1007/s00216-017-0721-z
M3 - Article
C2 - 29138881
AN - SCOPUS:85033732427
SN - 1618-2642
VL - 410
SP - 325
EP - 335
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 2
ER -