TY - JOUR
T1 - A gene cluster for 6-deoxy-L-talan synthesis in Actinobacillus actinomycetemcomitans
AU - Nakano, Yoshio
AU - Yoshida, Yasuo
AU - Yamashita, Yoshihisa
AU - Koga, Toshihiko
N1 - Funding Information:
This work was supported in part by grants-in-aid of scientific research Nos. 07557136 and 08457572 from the Ministry of Education, Science, Sports and Culture, Tokyo, Japan, and by a research grant from the Fund for Comprehensive Research on Aging and Health.
PY - 1998/11/8
Y1 - 1998/11/8
N2 - The serotype c antigen of Actinobacillus actinomycetemcomitans consists of 6-deoxy-L-talose. A gene cluster involved in the synthesis of serotype- specific polysaccharide antigen was cloned from the chromosomal DNA of A. actinomycetemcomitans NCTC 9710 (serotype c). This cluster consisted of 17 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype c-specific antibody when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with a similar duster from A. actinomycetemcomitans Y4 (serotype b), which produces a polysaccharide consisting of L-rhamnose and D-fucose, revealed that a 5.7 kb region containing seven genes in the cluster from strain Y4 was replaced by a 3.8 kb region containing three genes in strain NCTC 9710. The results suggest that these region, as well as dTDP-6-deoxy-L-talose-forming dTDP-4- keto-L-rhamnose reductase, is essential to the production of extracellular polysaccharide specific to serotype c.
AB - The serotype c antigen of Actinobacillus actinomycetemcomitans consists of 6-deoxy-L-talose. A gene cluster involved in the synthesis of serotype- specific polysaccharide antigen was cloned from the chromosomal DNA of A. actinomycetemcomitans NCTC 9710 (serotype c). This cluster consisted of 17 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype c-specific antibody when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with a similar duster from A. actinomycetemcomitans Y4 (serotype b), which produces a polysaccharide consisting of L-rhamnose and D-fucose, revealed that a 5.7 kb region containing seven genes in the cluster from strain Y4 was replaced by a 3.8 kb region containing three genes in strain NCTC 9710. The results suggest that these region, as well as dTDP-6-deoxy-L-talose-forming dTDP-4- keto-L-rhamnose reductase, is essential to the production of extracellular polysaccharide specific to serotype c.
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U2 - 10.1016/S0167-4781(98)00174-2
DO - 10.1016/S0167-4781(98)00174-2
M3 - Article
C2 - 9805002
AN - SCOPUS:0032497817
SN - 0167-4781
VL - 1442
SP - 409
EP - 414
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 2-3
ER -