A distinct binding mode of archaeal ribonuclease P proteins to RNA

Masato Ishihara, Etsuko Nishimoto, Shoji Yamashita, Yoshimitsu Kakuta, Makoto Kimura

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


The ribonuclease P (RNase P) in the hyperthermophilic archaeon Pyrococcus horikoshii comprises RNA (PhopRNA) and five proteins. We analyzed the RNA binding mode of the protein, using a pair of complementary fluorescence-labeled oligoribonucleotides. Fluorescence resonance energy transfer (FRET)-based assays suggested that the RNase P proteins assist PhopRNA in attaining a functionally active conformation via a distinct mode of binding.

Original languageEnglish
Pages (from-to)2335-2337
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Issue number12
Publication statusPublished - 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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