TY - JOUR
T1 - A defective prostaglandin E synthase could affect egg formation in the silkworm Bombyx mori
AU - Yamamoto, Kohji
AU - Tsubota, Takuya
AU - Uno, Tomohide
AU - Tsujita, Yutaro
AU - Yokota, Shingo
AU - Sezutsu, Hideki
AU - Mita, Kazuei
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research [KAKENHI , 17K19272 and 16KK0172 ] from the Ministry of Education, Culture, Sports, Science and Technology of Japan . Silkworm strains used in this study were supported by the National Bio-Resource Project (NBRP) of the MEXT, Japan .
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2020/1/8
Y1 - 2020/1/8
N2 - We had previously reported a prostaglandin E synthase (bmPGES) in the silkworm Bombyx mori that catalyzes the isomerization of PGH2 to PGE2. The present study aimed to provide a genome-editing characterization of bmPGES in B. mori. Results showed bmPGES gene disruption to result in a reduced content of PGE2. The change affected the expression of chorion genes and egg formation in silkworms. Collectively, the results indicated that bmPGES could be involved in reproduction of B. mori. Therefore, this study provides insights into the physiological role of bmPGES and PGE2 in silkworms.
AB - We had previously reported a prostaglandin E synthase (bmPGES) in the silkworm Bombyx mori that catalyzes the isomerization of PGH2 to PGE2. The present study aimed to provide a genome-editing characterization of bmPGES in B. mori. Results showed bmPGES gene disruption to result in a reduced content of PGE2. The change affected the expression of chorion genes and egg formation in silkworms. Collectively, the results indicated that bmPGES could be involved in reproduction of B. mori. Therefore, this study provides insights into the physiological role of bmPGES and PGE2 in silkworms.
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U2 - 10.1016/j.bbrc.2019.10.121
DO - 10.1016/j.bbrc.2019.10.121
M3 - Article
C2 - 31668810
AN - SCOPUS:85074333780
SN - 0006-291X
VL - 521
SP - 347
EP - 352
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -