TY - JOUR
T1 - A cytoplasmic AAA family peroxin, Pex1p, interacts with Pex6p
AU - Tamura, Shigehiko
AU - Shimozawa, Nobuyuki
AU - Suzuki, Yasuyuki
AU - Tsukamoto, Toshiro
AU - Osumi, Takashi
AU - Fujiki, Yukio
N1 - Funding Information:
We thank T. Sakaguchi and N. Matsumoto for technical assistance and the members of the Fujiki laboratory for comments. This work was supported in part by CREST grant (to Y.F.) from the Japan Science and Technology Corporation and by Grants-in-Aid for Scien-ti®c Research (07408016, 08249232 and 08557011 to Y.F.) from The Ministry of Education, Science, Sports, and Culture.
PY - 1998/4/28
Y1 - 1998/4/28
N2 - Human PEX1 (HsPEX1) is the causative gene for peroxisome-deficiency disorders such as Zellweger syndrome of complementation group I, encoding the peroxin, Pex1p, a member of AAA family. Pex1p tagged with an epitope flag was expressed in wild-type Chinese hamster ovary (CHO) cell, CHO-K1. Pex1p was localized in the cytoplasm, as assessed by immunofluorescent microscopy. Cell-lysate of HsPEX1-transfected CHO-K1 was incubated with in vitro synthesized 35S-labelled Pex6p, an AAA family peroxin. Immunoprecipitation of Pex1p using anti-Pex1p antibody resulted in concomitant recovery of 35S-Pex6p. Conversely, 35S-Pex1p was obtained in immunoprecipitate from CHO-K1 expressing human Pex6p, using anti-Pex6p antibody. These results strongly suggest that Pex1p and Pex6p interact with each other.
AB - Human PEX1 (HsPEX1) is the causative gene for peroxisome-deficiency disorders such as Zellweger syndrome of complementation group I, encoding the peroxin, Pex1p, a member of AAA family. Pex1p tagged with an epitope flag was expressed in wild-type Chinese hamster ovary (CHO) cell, CHO-K1. Pex1p was localized in the cytoplasm, as assessed by immunofluorescent microscopy. Cell-lysate of HsPEX1-transfected CHO-K1 was incubated with in vitro synthesized 35S-labelled Pex6p, an AAA family peroxin. Immunoprecipitation of Pex1p using anti-Pex1p antibody resulted in concomitant recovery of 35S-Pex6p. Conversely, 35S-Pex1p was obtained in immunoprecipitate from CHO-K1 expressing human Pex6p, using anti-Pex6p antibody. These results strongly suggest that Pex1p and Pex6p interact with each other.
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U2 - 10.1006/bbrc.1998.8522
DO - 10.1006/bbrc.1998.8522
M3 - Article
C2 - 9588209
AN - SCOPUS:0032576976
SN - 0006-291X
VL - 245
SP - 883
EP - 886
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -