TY - JOUR
T1 - A cyanobacterial non-coding RNA, Yfr1, is required for growth under multiple stress conditions
AU - Nakamura, Takahiro
AU - Naito, Kumiko
AU - Yokota, Naoto
AU - Sugita, Chieko
AU - Sugita, Mamoru
N1 - Funding Information:
We thank Dr. Yukihiro Kabeya for valuable discussion, Professor Tatsuo Omata for culturing of Synechococcus cells under high or low CO2 conditions, and Dr. Hideo Iwasaki for array analysis. The oligonucleotide array for S. elongatus PCC 6301 was kindly provided by Professor Takao Kondo. This work was supported by a Grand-in-aid for Scientific Research on Priority Area ‘Genome Biology’ (13206027 to M.S.) of the Ministry of Education, Culture, Sports, Science, and Technology, Japan. T.N. was a recipient of a Japan Society for the Promotion of Science (JSPS) postdoctoral fellowship.
PY - 2007/9
Y1 - 2007/9
N2 - Small, regulatory, non-coding RNA (ncRNA) is involved in various cell functions in both prokaryotes and eukaryotes. However, information on ncRNA in cyanobacteria is still scarce. We studied ncRNA genes by computational screening to compare the intergenic regions of the Synechococcus elongatus PCC 6301 genome with the genomes of three freshwater cyanobacteria. We identified an ncRNA gene in S. elongatus, which has been previously described as yfr1 in marine cyanobacteria. The S. elongatus yfr1 gene is 65 nucleotides long and is positioned between guaB and trxA. We found a high conservation of the yfr1 gene in most cyanobacterial lineages. A yfr1-deficient mutant showed reduced growth under various stress conditions, e.g. oxidative stress and high salt stress conditions, and showed unusual accumulation of sbtA mRNA. A gel shift assay demonstrated interaction of the Yfr1 RNA with sbtA mRNA in vitro. This suggests that the sbtA transcript is a target RNA for the Yfr1 RNA.
AB - Small, regulatory, non-coding RNA (ncRNA) is involved in various cell functions in both prokaryotes and eukaryotes. However, information on ncRNA in cyanobacteria is still scarce. We studied ncRNA genes by computational screening to compare the intergenic regions of the Synechococcus elongatus PCC 6301 genome with the genomes of three freshwater cyanobacteria. We identified an ncRNA gene in S. elongatus, which has been previously described as yfr1 in marine cyanobacteria. The S. elongatus yfr1 gene is 65 nucleotides long and is positioned between guaB and trxA. We found a high conservation of the yfr1 gene in most cyanobacterial lineages. A yfr1-deficient mutant showed reduced growth under various stress conditions, e.g. oxidative stress and high salt stress conditions, and showed unusual accumulation of sbtA mRNA. A gel shift assay demonstrated interaction of the Yfr1 RNA with sbtA mRNA in vitro. This suggests that the sbtA transcript is a target RNA for the Yfr1 RNA.
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U2 - 10.1093/pcp/pcm098
DO - 10.1093/pcp/pcm098
M3 - Article
C2 - 17664182
AN - SCOPUS:34548758619
SN - 0032-0781
VL - 48
SP - 1309
EP - 1318
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 9
ER -
