TY - JOUR
T1 - A comparative study and phage typing of silage-making Lactobacillus bacteriophages
AU - Doi, Katsumi
AU - Zhang, Ye
AU - Nishizaki, Yousuke
AU - Umeda, Akiko
AU - Ohmomo, Sadahiro
AU - Ogata, Seiya
N1 - Funding Information:
This work was supported in part by a Grant-m-Aid for Scientific Research (A, no. 07556094) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by the research foundation from the Ministry of Agriculture, Forestry and Fisheries of Japan. M. Ohara provided language assistance.
PY - 2003
Y1 - 2003
N2 - To investigate basic characteristics of 10 virulent phages active on silage-making lactobacilli, morphological properties, host ranges, protein composition and genome characterization were separated into five groups based on host ranges and basic properties. The seven phages of groups I, II and V were active on Lactobacillus plantarum and Lactobacillus pentosus. Phage φPY4 (group III) infected both L. casei and Lactobacillus rhamnosus. Phage φPY5 (group IV) specifically infected Lactobacillus casei. Morphologically, three phages of groups I belonged to the Myoviridae family, while seven other phages of groups II, III and V belonged to the Siphoviridae family. SDS-PAGE profiles, restriction analysis, G+C contents of DNA and Dot blot hybridization revealed a high degree of homology in each group. Clustering derived from host range analysis was closely related to results of DNA and protein analyses. These phages may be applicable to phage typing for silage-making lactobacilli.
AB - To investigate basic characteristics of 10 virulent phages active on silage-making lactobacilli, morphological properties, host ranges, protein composition and genome characterization were separated into five groups based on host ranges and basic properties. The seven phages of groups I, II and V were active on Lactobacillus plantarum and Lactobacillus pentosus. Phage φPY4 (group III) infected both L. casei and Lactobacillus rhamnosus. Phage φPY5 (group IV) specifically infected Lactobacillus casei. Morphologically, three phages of groups I belonged to the Myoviridae family, while seven other phages of groups II, III and V belonged to the Siphoviridae family. SDS-PAGE profiles, restriction analysis, G+C contents of DNA and Dot blot hybridization revealed a high degree of homology in each group. Clustering derived from host range analysis was closely related to results of DNA and protein analyses. These phages may be applicable to phage typing for silage-making lactobacilli.
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U2 - 10.1263/jbb.95.518
DO - 10.1263/jbb.95.518
M3 - Article
C2 - 16233449
AN - SCOPUS:0037832668
SN - 1389-1723
VL - 95
SP - 518
EP - 525
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 5
ER -