A cDNA resource for gene expression studies of a hemichordate, ptychodera flava

Kuni Tagawa; Asuka Arimito; Akane Sasaki; Mizuki Izumi; Satoko Fujita; Tom Humphreys; Asao Fujiyama; Hiroshi Kagoshima; Tadasu Shin-I; Yuji Kohara; Nori Satoh; Takeshi Kawashima

doi:10.2108/zs130262

A cDNA resource for gene expression studies of a hemichordate, ptychodera flava

Kuni Tagawa, Asuka Arimito, Akane Sasaki, Mizuki Izumi, Satoko Fujita, Tom Humphreys, Asao Fujiyama, Hiroshi Kagoshima, Tadasu Shin-I, Yuji Kohara, Nori Satoh, Takeshi Kawashima

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Recent investigations into the evolution of deuterostomes and the origin of chordates have paid considerable attention to hemichordates (acorn worms), as hemichordates and echinoderms are the closest chordate relatives. The present study prepared cDNA libraries from Ptychodera flava, to study expression and function of genes involved in development of the hemichordate body plan. Expressed sequence tag (EST) analyses of nine cDNA libraries yielded 18,832 cloned genes expressed in eggs, 18,739 in blastulae, 18,539 in gastrulae, 18,811 in larvae, 18,978 in juveniles, 11,802 in adult proboscis, 17,259 in stomochord, 11,886 in gills, and 11,580 in liver, respectively. A set of 34,159 uni-gene clones of P. flava was obtained. This cDNA resource will be valuable for studying temporal and spatial expression of acorn worm genes during development.

Original language	English
Pages (from-to)	414-420
Number of pages	7
Journal	Zoological science
Volume	31
Issue number	7
DOIs	https://doi.org/10.2108/zs130262
Publication status	Published - Jul 2014
Externally published	Yes

All Science Journal Classification (ASJC) codes

Medicine(all)

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10.2108/zs130262

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title = "A cDNA resource for gene expression studies of a hemichordate, ptychodera flava",

abstract = "Recent investigations into the evolution of deuterostomes and the origin of chordates have paid considerable attention to hemichordates (acorn worms), as hemichordates and echinoderms are the closest chordate relatives. The present study prepared cDNA libraries from Ptychodera flava, to study expression and function of genes involved in development of the hemichordate body plan. Expressed sequence tag (EST) analyses of nine cDNA libraries yielded 18,832 cloned genes expressed in eggs, 18,739 in blastulae, 18,539 in gastrulae, 18,811 in larvae, 18,978 in juveniles, 11,802 in adult proboscis, 17,259 in stomochord, 11,886 in gills, and 11,580 in liver, respectively. A set of 34,159 uni-gene clones of P. flava was obtained. This cDNA resource will be valuable for studying temporal and spatial expression of acorn worm genes during development.",

author = "Kuni Tagawa and Asuka Arimito and Akane Sasaki and Mizuki Izumi and Satoko Fujita and Tom Humphreys and Asao Fujiyama and Hiroshi Kagoshima and Tadasu Shin-I and Yuji Kohara and Nori Satoh and Takeshi Kawashima",

year = "2014",

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doi = "10.2108/zs130262",

language = "English",

volume = "31",

pages = "414--420",

journal = "Zoological science",

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T1 - A cDNA resource for gene expression studies of a hemichordate, ptychodera flava

AU - Tagawa, Kuni

AU - Arimito, Asuka

AU - Sasaki, Akane

AU - Izumi, Mizuki

AU - Fujita, Satoko

AU - Humphreys, Tom

AU - Fujiyama, Asao

AU - Kagoshima, Hiroshi

AU - Shin-I, Tadasu

AU - Kohara, Yuji

AU - Satoh, Nori

AU - Kawashima, Takeshi

PY - 2014/7

Y1 - 2014/7

N2 - Recent investigations into the evolution of deuterostomes and the origin of chordates have paid considerable attention to hemichordates (acorn worms), as hemichordates and echinoderms are the closest chordate relatives. The present study prepared cDNA libraries from Ptychodera flava, to study expression and function of genes involved in development of the hemichordate body plan. Expressed sequence tag (EST) analyses of nine cDNA libraries yielded 18,832 cloned genes expressed in eggs, 18,739 in blastulae, 18,539 in gastrulae, 18,811 in larvae, 18,978 in juveniles, 11,802 in adult proboscis, 17,259 in stomochord, 11,886 in gills, and 11,580 in liver, respectively. A set of 34,159 uni-gene clones of P. flava was obtained. This cDNA resource will be valuable for studying temporal and spatial expression of acorn worm genes during development.

AB - Recent investigations into the evolution of deuterostomes and the origin of chordates have paid considerable attention to hemichordates (acorn worms), as hemichordates and echinoderms are the closest chordate relatives. The present study prepared cDNA libraries from Ptychodera flava, to study expression and function of genes involved in development of the hemichordate body plan. Expressed sequence tag (EST) analyses of nine cDNA libraries yielded 18,832 cloned genes expressed in eggs, 18,739 in blastulae, 18,539 in gastrulae, 18,811 in larvae, 18,978 in juveniles, 11,802 in adult proboscis, 17,259 in stomochord, 11,886 in gills, and 11,580 in liver, respectively. A set of 34,159 uni-gene clones of P. flava was obtained. This cDNA resource will be valuable for studying temporal and spatial expression of acorn worm genes during development.

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JO - Zoological science

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A cDNA resource for gene expression studies of a hemichordate, ptychodera flava

Abstract

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