TY - JOUR
T1 - A carotenoid-binding protein (CBP) plays a crucial role in cocoon pigmentation of silkworm (Bombyx mori) larvae
AU - Tabunoki, Hiroko
AU - Higurashi, Satoshi
AU - Ninagi, Osamu
AU - Fujii, Hiroshi
AU - Banno, Yutaka
AU - Nozaki, Masashi
AU - Kitajima, Mika
AU - Miura, Nami
AU - Atsumi, Shogo
AU - Tsuchida, Kozo
AU - Maekawa, Hideaki
AU - Sato, Ryoichi
N1 - Funding Information:
This work was supported in part by the Sasakawa Scientific Research Grant from the Japan Science Society. We are indebted to Dr. Toru Yokoyama and Dr. Yuichiro Tabunoki for critical reading of the manuscript.
PY - 2004/6/4
Y1 - 2004/6/4
N2 - We examined the role of carotenoid-binding protein (CBP) in yellow cocoon pigmentation. First, using yellow or white cocoon races, we investigated the linkage between the yellow pigmentation and CBP expression. CBP was expressed only in the silk gland of the yellow cocoon races, which utilize carotenoids for cocoon pigmentation. Furthermore, CBP expression in the silk glands of day 1-7 fifth instar larvae matched the period of carotenoid uptake into the silk gland. Finally, we gave double-stranded CBP RNA to Bombyx mori (B. mori) larvae to induce RNA interference. The significantly reduced expression of CBP in the silk gland of fifth instar larva was confirmed on day 4 and a decrease in yellow pigmentation was observed in the cocoon. We showed that CBP plays a key role in the yellow cocoon pigmentation caused by carotenoids.
AB - We examined the role of carotenoid-binding protein (CBP) in yellow cocoon pigmentation. First, using yellow or white cocoon races, we investigated the linkage between the yellow pigmentation and CBP expression. CBP was expressed only in the silk gland of the yellow cocoon races, which utilize carotenoids for cocoon pigmentation. Furthermore, CBP expression in the silk glands of day 1-7 fifth instar larvae matched the period of carotenoid uptake into the silk gland. Finally, we gave double-stranded CBP RNA to Bombyx mori (B. mori) larvae to induce RNA interference. The significantly reduced expression of CBP in the silk gland of fifth instar larva was confirmed on day 4 and a decrease in yellow pigmentation was observed in the cocoon. We showed that CBP plays a key role in the yellow cocoon pigmentation caused by carotenoids.
UR - http://www.scopus.com/inward/record.url?scp=2942566457&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2942566457&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2004.04.067
DO - 10.1016/j.febslet.2004.04.067
M3 - Article
C2 - 15178318
AN - SCOPUS:2942566457
SN - 0014-5793
VL - 567
SP - 175
EP - 178
JO - FEBS Letters
JF - FEBS Letters
IS - 2-3
ER -