During the maintenance of the wild silkworm, Bombyx mandarina, a mutant phenotype exhibiting translucent skin was identified. Based on the crossing experiments with the domesticated silkworm, Bombyx mori, we found that the mutant was controlled by molybdenum cofactor sulfurase (MoCoS) gene. We designated the mutant "Ozaki's translucent" (ogZ). We found a 2.1-kb deletion containing the transcription initiation site, exons 1 and 2, and the 5′ end of exon 3 of the MoCoS gene. The transcript of the MoCoS gene was not detected in the ogZ homozygote. We concluded that ogZ is a complete loss-of-function allele generated by a disruption of the MoCoS gene.
All Science Journal Classification (ASJC) codes
- Molecular Biology