1-methyl-4-phenylpyridinium (MPP+) inhibits mitochondrial oxygen consumption mediated by succinate as well as malate in rat pheochromocytoma PC12 cells

E. Hasegawa; H. Asagami; D. Kang; S. Minakami; K. Takeshige

1-methyl-4-phenylpyridinium (MPP⁺) inhibits mitochondrial oxygen consumption mediated by succinate as well as malate in rat pheochromocytoma PC12 cells

E. Hasegawa, H. Asagami, D. Kang, S. Minakami, K. Takeshige

Department of Basic Medicine

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

When rat pheochromocytoma PC12 cells are cultured with 1 mM 1-methyl-4-phenylpyridinium (MPP⁺), the number of viable cells decreases to one third in 4 days while the number increases ten-fold without MPP⁺. Oxygen consumption by mitochondria in the presence of malate is inhibited about 80% by the treatment of the cells with MPP⁺ for 4 days. Unexpectedly, succinate-dependent oxygen consumption is also inhibited to essentially the same extent as malate-dependent one. These results suggest that the impairment of the respiration mediated by succinate as well as malate is important as a mechanism of MPP⁺-induced cell death.

Original language	English
Pages (from-to)	409-413
Number of pages	5
Journal	Biochemistry and Molecular Biology International
Volume	35
Issue number	2
Publication status	Published - 1995

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Genetics

Cite this

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title = "1-methyl-4-phenylpyridinium (MPP+) inhibits mitochondrial oxygen consumption mediated by succinate as well as malate in rat pheochromocytoma PC12 cells",

abstract = "When rat pheochromocytoma PC12 cells are cultured with 1 mM 1-methyl-4-phenylpyridinium (MPP+), the number of viable cells decreases to one third in 4 days while the number increases ten-fold without MPP+. Oxygen consumption by mitochondria in the presence of malate is inhibited about 80% by the treatment of the cells with MPP+ for 4 days. Unexpectedly, succinate-dependent oxygen consumption is also inhibited to essentially the same extent as malate-dependent one. These results suggest that the impairment of the respiration mediated by succinate as well as malate is important as a mechanism of MPP+-induced cell death.",

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AU - Kang, D.

AU - Minakami, S.

AU - Takeshige, K.

PY - 1995

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AB - When rat pheochromocytoma PC12 cells are cultured with 1 mM 1-methyl-4-phenylpyridinium (MPP+), the number of viable cells decreases to one third in 4 days while the number increases ten-fold without MPP+. Oxygen consumption by mitochondria in the presence of malate is inhibited about 80% by the treatment of the cells with MPP+ for 4 days. Unexpectedly, succinate-dependent oxygen consumption is also inhibited to essentially the same extent as malate-dependent one. These results suggest that the impairment of the respiration mediated by succinate as well as malate is important as a mechanism of MPP+-induced cell death.

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1-methyl-4-phenylpyridinium (MPP⁺) inhibits mitochondrial oxygen consumption mediated by succinate as well as malate in rat pheochromocytoma PC12 cells

Abstract

All Science Journal Classification (ASJC) codes

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