嫌気‐無酸素連続回分スクリーニング法による脱窒脱リン菌単離の試み

A brandnew screening equipment was proposed to isolate the denitrifying phosphate-accumulating organisms. A screening work was carried out under the altemating anaerobic-anoxic condition. At only anaerobic phase, acetate was used as a sole organic matter. At the anoxic phase, nitrate as a final electron accepter and no organic matters were added. Since the inside of equipment was washed with distilled water at the shifting phase from each condition to the other condition, acetate and nitrate did not exist simultaneously during the work. As a result of these works using activated sludge in a municipal waste water treatment plant, three kinds of pure cultures of <I>Stenotrophomonas acidaminiphila</I> closely related species, <I>Pseudomonas fulva</I> closely related species and <I>Acinetobacter johnsonii</I> closely related species were obtained. All of isolated bacteria were able to accumulate polyphosphate and PHB, and had the phosphate removal activity under unique conditions such as altemating anaerobic-anoxic conditions. By microscopy with a staining method it is reasonable to suppose qualitatively that these bacteria are responsible for enhanced biological phosphorus removal.