Abstract
<p>Based on the structure of invertebrate-type lyzozyme, <i>Venerupis philippinarum</i> lysozyme (<i>Vp</i>L), we designed Asn46Asp/Asp52Ser or Asn46Glu/Asp52Ser hen egg white lysozyme (HEWL) mutants. Both lysozyme mutants possessed lysozyme acitivity like <i>Vp</i>L and HEWL. Although Asn46Glu/Asp52Ser mutant formed a glycosyl adduct in the reaction with the N-acetylglucosamine oligomer, however, the turnover rate of estimated from the glycosyl formation and decomposition rates was only 20% of the observed hydrolysis rate of the substrate. From these results, we discussed the catalytic mechanism of lysozymes.</p>
| Translated title of the contribution | Catalytic Mechanism of Lysozyme Based on the Structures of Invertebrate-type Lysozyme and Chicken-type Lysozyme |
|---|---|
| Original language | Japanese |
| Pages (from-to) | 140-143 |
| Number of pages | 4 |
| Journal | 生物物理 |
| Volume | 57 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2017 |