ウシ血清アルブミン固定化シートを用いたエンドウの根滲出物の可視化

Translated title of the contribution: Visualization of Root Exudates from Pea Roots Using Sheets with Immobilized Bovine Serum Albumin

Takeshi Onodera, Haruna Miyazaki, Masaru Nakayasu, Nozomu Sakurai, Akifumi Sugiyama

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In leguminous plants, rhizobium symbiotically forms nodules, which are responsible for nitrogen fixation and obtaining nutrients necessary for growth. Plant roots are thought to secrete chemicals that signal the formation of nodules. In this study, we attempted to visualize the concentration distribution of the exudates from pea roots by using the phenomenon that the fluorescence intensity decreases due to the interaction of bovine serum albumin (BSA) with phenolic compounds. BSA was immobilized on a glass microfiber filter (sheets with immobilized BSA), and the quenched fluorescence of BSA due to the interaction with root exudates was visualized. The correlation coefficient between the quenched fluorescence intensity per sheet and the amount of kaempferol in hydrolyzed sample recovered from six sheets with immobilized BSA was 0.8 (p < 0.05), indicating a high correlation.

Translated title of the contributionVisualization of Root Exudates from Pea Roots Using Sheets with Immobilized Bovine Serum Albumin
Original languageJapanese
Pages (from-to)133-138
Number of pages6
JournalIEEJ Transactions on Sensors and Micromachines
Volume142
Issue number7
DOIs
Publication statusPublished - 2022

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Electrical and Electronic Engineering

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